Human V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) ELISA Kit
The Human V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) ELISA Kit is a cutting-edge tool for the precise measurement of KRAS levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it perfect for various research applications.KRAS is a pivotal oncogene that plays a crucial role in cancer development and progression. Abnormal KRAS signaling is often found in various types of cancers, making it a promising target for cancer treatment and personalized medicine.
The Human KRAS ELISA Kit enables researchers to analyze KRAS levels in different samples, providing valuable insights into cancer biology and potential therapeutic strategies.Overall, the Human KRAS ELISA Kit is a valuable tool for studying KRAS biology, identifying potential therapeutic targets, and advancing cancer research. Its high performance and reliability make it a must-have for researchers interested in unraveling the complexities of cancer biology and developing novel treatment approaches.
Product Name:
Human V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
