The Human VDR (Vitamin D Receptor) CLIA Kit is specifically designed for the precise measurement of VDR levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit guarantees trustworthy and consistent results, perfect for various research purposes.The Vitamin D Receptor is a key protein that plays a vital role in the regulation of vitamin D metabolism and function. It is essential for maintaining calcium homeostasis, bone health, and immune system function.
Variations in VDR levels have been linked to various diseases such as osteoporosis, autoimmune disorders, and certain cancers, highlighting its importance as a biomarker for studying and potentially treating these conditions.Don't miss out on the opportunity to accurately quantify VDR levels with the Human VDR CLIA Kit, and enhance your research efforts in understanding the intricate role of vitamin D in human health and disease.
Product Name:
Human VDR (Vitamin D Receptor) CLIA Kit
SKU:
HUES01096
Target:
Human VDR (Vitamin D Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VDR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VDR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VDR. You can calculate the concentration of Human VDR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
94-107
100-117
Average (%)
98
99
107
1:4
Range (%)
93-105
92-105
102-119
Average (%)
98
98
109
1:8
Range (%)
86-98
90-104
93-109
Average (%)
92
96
100
1:16
Range (%)
97-110
99-114
100-117
Average (%)
103
105
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
93-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
394.38
844.69
2809.83
432.84
805.2
2605.02
Standard deviation
46.1
75.01
303.74
47.66
91.87
222.21
C V (%)
11.69
8.88
10.81
11.01
11.41
8.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VDR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VDR in samples. No significant cross-reactivity or interference between Human VDR and analogues was observed.
