The Human VDR (Vitamin D Receptor) ELISA Kit is specifically designed for the precise measurement of Vitamin D receptor levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and accuracy, ensuring dependable and reproducible results for various research purposes.The Vitamin D receptor is a key protein that plays a vital role in regulating the effects of Vitamin D in the body, impacting various biological processes such as calcium metabolism, immune function, and cell growth.
Dysregulation of the Vitamin D receptor has been associated with numerous health conditions, including osteoporosis, autoimmune diseases, and certain cancers, making it an essential marker for studying these diseases and potential therapeutic interventions.Overall, the Human VDR ELISA Kit from AssayGenie provides researchers with a powerful tool for investigating the role of Vitamin D receptor in health and disease, offering valuable insights into its mechanisms and potential clinical implications.
Product Name:
Human VDR (Vitamin D Receptor) ELISA Kit
SKU:
HUES02964
Target:
Human VDR (Vitamin D Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VDR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VDR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VDR. You can calculate the concentration of Human VDR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
91-104
96-114
Average (%)
97
97
104
1:4
Range (%)
91-103
81-94
88-98
Average (%)
96
86
93
1:8
Range (%)
88-100
80-93
82-96
Average (%)
95
86
89
1:16
Range (%)
88-99
82-97
81-93
Average (%)
94
89
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.85
5.89
18.83
1.82
6.22
17.53
Standard deviation
0.1
0.35
0.69
0.1
0.35
0.82
C V (%)
5.41
5.94
3.66
5.49
5.63
4.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VDR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VDR in samples. No significant cross-reactivity or interference between Human VDR and analogues was observed.
