Human VEGF-B (Vascular Endothelial Cell Growth Factor B) ELISA Kit
The Human VEGF-B (Vascular Endothelial Cell Growth Factor-B) ELISA Kit is specifically designed for the accurate measurement of VEGF-B levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for various research applications.VEGF-B is a key protein involved in angiogenesis, contributing to the growth and development of blood vessels. It plays a crucial role in various physiological and pathological processes, including cardiovascular diseases, cancer, and other related conditions.
By studying VEGF-B levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for treatment.Overall, the Human VEGF-B ELISA Kit offers a powerful tool for investigating VEGF-B biology and its implications in disease pathology, paving the way for the development of innovative treatments and interventions.
Product Name:
Human VEGF-B (Vascular Endothelial Cell Growth Factor B) ELISA Kit
SKU:
HUES03065
Target:
Human VEGF-B (Vascular Endothelial Cell Growth Factor B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-B. You can calculate the concentration of Human VEGF-B in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
95-107
95-108
Average (%)
95
101
101
1:4
Range (%)
88-104
78-92
87-100
Average (%)
95
85
94
1:8
Range (%)
88-102
79-90
87-98
Average (%)
94
85
92
1:16
Range (%)
89-99
81-95
88-103
Average (%)
94
88
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.07
102.94
400.36
51.34
106.2
365.76
Standard deviation
2.75
4.67
13.05
3.14
4.59
16.06
C V (%)
5.38
4.54
3.26
6.12
4.32
4.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VEGF-B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGF-B in samples. No significant cross-reactivity or interference between Human VEGF-B and analogues was observed.
