Human VEGF-C (Vascular Endothelial Growth Factor C) CLIA Kit
The Human VEGF-C (Vascular Endothelial Growth Factor-C) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of VEGF-C levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it the perfect choice for a wide range of research studies.VEGF-C is a vital protein involved in promoting the growth of blood vessels and influencing cell proliferation.
Its crucial role in various health conditions including cancer, cardiovascular diseases, and neurodegenerative disorders has made it a key biomarker for research and therapeutic development. By accurately measuring VEGF-C levels, researchers can gain valuable insights into disease mechanisms and potential treatment strategies.
Product Name:
Human VEGF-C (Vascular Endothelial Growth Factor C) CLIA Kit
SKU:
HUES00911
Target:
Human VEGF-C (Vascular Endothelial Growth Factor C)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VEGF-C. You can calculate the concentration of Human VEGF-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
92-105
101-115
Average (%)
91
99
107
1:4
Range (%)
98-114
92-108
84-97
Average (%)
104
100
90
1:8
Range (%)
101-120
101-113
96-109
Average (%)
110
107
101
1:16
Range (%)
94-108
93-108
94-109
Average (%)
101
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-110
105
EDTA plasma (n=5)
97-112
103
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.39
308.54
852.34
97.94
306.4
886.1
Standard deviation
11.89
35.3
57.79
9.19
24.05
100.4
C V (%)
11.61
11.44
6.78
9.38
7.85
11.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VEGF-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGF-C in samples. No significant cross-reactivity or interference between Human VEGF-C and analogues was observed.
