Human VIP (Vasoactive Intestinal Peptide) ELISA Kit
The Human VIP (Vasoactive Intestinal Peptide) ELISA Kit is specifically designed for the precise measurement of VIP levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an excellent choice for various research applications.VIP is a vital neuropeptide that plays a critical role in regulating numerous physiological functions, including smooth muscle relaxation, immune response modulation, and hormone secretion.
Dysregulation of VIP has been linked to various disorders such as inflammation, autoimmune diseases, and neurological disorders, highlighting its significance as a biomarker for studying and developing treatments for these conditions.In conclusion, the Human VIP ELISA Kit is a valuable tool for researchers looking to study the role of VIP in human health and disease, offering reliable and precise measurements for insightful and impactful research findings.
Product Name:
Human VIP (Vasoactive Intestinal Peptide) ELISA Kit
SKU:
HUES03058
Target:
Human VIP (Vasoactive Intestinal Peptide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VIP. During the reaction, VIP in the sample or standard competes with a fixed amount of VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VIP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
93-107
92-107
Average (%)
94
98
99
1:4
Range (%)
83-97
92-104
99-114
Average (%)
90
97
104
1:8
Range (%)
87-100
93-105
97-112
Average (%)
94
98
103
1:16
Range (%)
83-96
86-102
95-111
Average (%)
90
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
86-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.5
74.3
194.5
22.7
75.3
202.1
Standard deviation
1.5
3.6
6.8
1.2
3.8
9.7
C V (%)
6.38
4.85
3.5
5.29
5.05
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of VIP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes VIP in samples. No significant cross-reactivity or interference between VIP and analogues was observed.
