The Human VTN (Vitronectin) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of vitronectin levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers superior sensitivity and specificity, ensuring accurate and consistent results for various research applications.Vitronectin is a key glycoprotein involved in cell adhesion and spreading, as well as in regulating the coagulation and fibrinolytic systems. It plays a crucial role in processes such as wound healing, tissue repair, and immune response modulation.
Abnormal levels of vitronectin have been associated with various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for studying these pathologies and developing potential treatment strategies.With its high-performance capabilities and versatility, the Human VTN CLIA Kit is an essential tool for researchers and clinicians seeking to investigate the role of vitronectin in health and disease. Trust in its precision and reliability to advance your research and diagnostic efforts with confidence.
Product Name:
Human VTN (Vitronectin) CLIA Kit
SKU:
HUES00643
Target:
Human VTN (Vitronectin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.56 ng/mL
Detection range:
0.94-60 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VTN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VTN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VTN. You can calculate the concentration of Human VTN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
94-109
96-108
Average (%)
98
101
101
1:4
Range (%)
90-105
98-113
90-104
Average (%)
98
104
96
1:8
Range (%)
99-114
86-98
89-101
Average (%)
106
92
94
1:16
Range (%)
85-96
92-105
95-106
Average (%)
90
100
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
108
EDTA plasma (n=5)
92-105
97
Cell culture media (n=5)
93-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.28
6.12
29.7
3.57
6.62
28.39
Standard deviation
0.34
0.46
2.71
0.35
0.55
2.49
C V (%)
10.37
7.52
9.12
9.8
8.31
8.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VTN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VTN in samples. No significant cross-reactivity or interference between Human VTN and analogues was observed.
