Human Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA Kit
The Human Wingless-Type MMTV Integration Site Family Member 5B (WNT5B) ELISA Kit is a cutting-edge tool for the precise measurement of WNT5B levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides accurate and consistent results, making it an indispensable asset for various research applications. WNT5B is a key member of the WNT signaling pathway, playing a crucial role in cell proliferation, differentiation, and migration. Dysregulation of WNT5B has been linked to various diseases including cancer, developmental disorders, and inflammatory conditions, highlighting its significance as a potential biomarker for disease research and therapeutic interventions.
By utilizing the Human WNT5B ELISA Kit, researchers can gain valuable insights into the role of WNT5B in disease pathology and identify novel therapeutic targets for precision medicine approaches. Don't miss out on the opportunity to enhance your research with this state-of-the-art ELISA kit from Assay Genie.
Product Name:
Human Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) ELISA Kit
Product Code:
HUDL03132
Size:
96 Assays
Target:
WNT5B
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
