The Human XOD (Xanthine Oxidase) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of Xanthine Oxidase levels in human serum, plasma, and cell culture supernatants. Xanthine Oxidase is an enzyme that plays a key role in the purine degradation pathway, generating reactive oxygen species that contribute to oxidative stress and inflammation.This ELISA kit offers reliable and reproducible results, making it an essential tool for researchers studying conditions related to oxidative stress, such as cardiovascular diseases, inflammatory disorders, and neurological diseases.
By accurately measuring Xanthine Oxidase levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Human XOD ELISA Kit from Assay Genie is a valuable tool for investigating the role of Xanthine Oxidase in human health and disease, providing researchers with the means to advance our understanding of oxidative stress-related pathologies and develop innovative treatments.
Product Name:
Human XOD (Xanthine Oxidase) ELISA Kit
SKU:
HUES03552
Target:
Human XOD (Xanthine Oxidase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human XOD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human XOD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human XOD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human XOD. You can calculate the concentration of Human XOD in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
91-103
85-99
Average (%)
96
97
91
1:4
Range (%)
94-106
79-90
87-101
Average (%)
99
85
93
1:8
Range (%)
95-109
84-97
85-100
Average (%)
100
91
91
1:16
Range (%)
89-104
85-98
83-95
Average (%)
96
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
0.85
4.85
0.49
0.79
4.64
Standard deviation
0.03
0.05
0.22
0.03
0.04
0.25
C V (%)
6.38
5.88
4.54
6.12
5.06
5.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human XOD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human XOD in samples. No significant cross-reactivity or interference between Human XOD and analogues was observed.
