The Human Zonulin ELISA Kit is a highly reliable tool for the precise measurement of zonulin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it well-suited for various research purposes.Zonulin is a key protein in regulating the permeability of the intestinal epithelial barrier. Dysregulation of zonulin has been associated with various gastrointestinal disorders, autoimmune diseases, and metabolic conditions, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and user-friendly protocol, the Human Zonulin ELISA Kit is an indispensable tool for researchers seeking to deepen their understanding of zonulin biology and its implications in health and disease.
Product Name:
Human Zonulin ELISA Kit
SKU:
HUES03536
Target:
Human Zonulin
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Zonulin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Zonulin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Zonulin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Zonulin. You can calculate the concentration of Human Zonulin in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
98-109
94-105
Average (%)
93
105
101
1:4
Range (%)
84-96
100-109
91-100
Average (%)
90
105
95
1:8
Range (%)
94-103
91-103
88-99
Average (%)
100
97
93
1:16
Range (%)
96-110
93-102
92-104
Average (%)
104
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
95
EDTA plasma (n=5)
87-99
91
Cell culture media (n=5)
91-99
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.46
5.57
22.64
2.31
6.06
23.45
Standard deviation
0.14
0.27
1.23
0.12
0.28
1.02
C V (%)
5.69
4.85
5.43
5.19
4.62
4.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Zonulin concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Zonulin in samples. No significant cross-reactivity or interference between Human Zonulin and analogues was observed.
Le et al.
Development of an Inflammation-Triggered In Vitro “Leaky Gut” Model Using Caco-2/HT29-MTX-E12 Combined with Macrophage-like THP-1 Cells or Primary Human-Derived Macrophages
Anti-Salmonella Defence and Intestinal Homeostatic Maintenance In Vitro of a Consortium Containing Limosilactobacillus fermentum 3872 and Ligilactobacillus salivarius 7247 Strains in Human, Porcine, and Chicken Enterocytes
