Description
| Product Name: | β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit |
| Product Code: | MAES0217 |
| Sample Type: | Serum, Plasma, Urine, Animal Tissue |
| Assay Time: | 60 min |
| Assay Type: | Quantitative |
| Synonyms: | β-HB |
| Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.01 mmol/L |
| Detection Range: | 0.01-2.00 mmol/L |
| Inter-assay CV: | 0.04 |
| Intra-assay CV: | 0.02 |
β- hydroxybutyrate dehydrogenase can catalyze the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm.
| Storage: | 2-8°C |
| Valid Period: | 12 months |
| Other Instruments Required: | Incubator, 50 kD Ultrafiltration tube, Microplate reader |
