The IBSP Polyclonal Antibody (CAB16220) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high specificity and sensitivity for detecting IBSP in human samples, making it an excellent choice for Western blot analysis.IBSP is known for its role in promoting the adhesion and differentiation of osteoblasts, cells responsible for building and maintaining bone tissue. By targeting IBSP, researchers can gain insights into the molecular mechanisms underlying bone development and turnover, as well as potential therapeutic strategies for bone-related disorders like osteoporosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IBSP Polyclonal Antibody
SKU:
CAB16220
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BSP, BNSP, SP-II, BSP-II, IBSP
Positive Sample:
U2OS, U-138MG, Rat lung
Cellular Localization:
Secreted.
Calculated MW:
35kDa
Observed MW:
35kDa
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
Purification Method
Affinity purification
Gene ID
3381
RRID
AB_2763675
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using IBSP Rabbit pAb (CAB16220) at 1:2500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
