The ID4 Antibody (CAB16229) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the inhibitor of DNA binding (ID) protein family. The encoded protein lacks DNA binding ability, and instead regulates gene expression through binding to and inhibiting basic helix-loop-helix transcription factors. This protein has been implicated in the regulation of diverse cellular processes that play a role in development and tumorigenesis. RRID AB_2769909 Gene ID 3400 Swiss Prot Synonym IDB4; bHLHb27; ID4
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ID4 Antibody
SKU:
CAB16229
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IDB4, bHLHb27, ID4
Positive Sample:
Mouse kidney
Cellular Localization:
Cytoplasm, Nucleoplasm, Nucleus.
Calculated MW:
17kDa
Observed MW:
20kDa
This gene encodes a member of the inhibitor of DNA binding (ID) protein family. The encoded protein lacks DNA binding ability, and instead regulates gene expression through binding to and inhibiting basic helix-loop-helix transcription factors. This protein has been implicated in the regulation of diverse cellular processes that play a role in development and tumorigenesis. RRID AB_2769909 Gene ID 3400 Swiss Prot Synonym IDB4; bHLHb27; ID4
Purification Method:
Affinity purification
Gene ID:
3400
RRID:
AB_2769909
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse kidney, using ID4 Rabbit pAb (CAB16229) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
