The IDS Antibody (CAB1857) is a high-quality antibody developed for reliable detection and analysis of target proteins. This enzyme is essential for the breakdown of glycosaminoglycans (GAGs) in the body, making it crucial for proper cellular function and development. The IDS antibody, produced in rabbits, is highly specific to human samples and has been validated for use in applications such as Western blotting.By targeting the IDS protein, this antibody enables researchers to detect and analyze IDS expression in various cell types, providing insight into its role in lysosomal storage disorders such as Hunter syndrome.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IDS Antibody
SKU:
CAB1857
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ID2S, MPS2, SIDS, IDS
Positive Sample:
HeLa, HepG2, A-549
Cellular Localization:
Lysosome.
Calculated MW:
62kDa
Observed MW:
60kDa
This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Purification Method
Affinity purification
Gene ID
3423
RRID
AB_2763892
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IDS Rabbit pAb (CAB1857) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using IDS Rabbit pAb (CAB1857) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using IDS Rabbit pAb (CAB1857) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using IDS Rabbit pAb (CAB1857) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
