The [KO Validated] IFITM3 Antibody (CAB13070) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications.IFITM3 is known for its role in restricting the entry of various viruses into host cells, making it a crucial component of the innate immune response. By targeting IFITM3, researchers can gain insights into the mechanisms of viral pathogenesis and potentially develop new antiviral strategies.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] IFITM3 Antibody
SKU:
CAB13070
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
1-8U, IP15, DSPA2b, M3
Positive Sample:
HeLa
Cellular Localization:
Cell Membrane, Late Endosome Membrane, Lysosome Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
15kDa
Observed MW:
15kDa
Interferon-induced transmembrane (IFITM) proteins are a family of interferon induced antiviral proteins. The family contains five members, including IFITM1, IFITM2 and IFITM3 and belong to the CD225 superfamily. The protein encoded by this gene restricts cellular entry by diverse viral pathogens, such as influenza A virus, Ebola virus and Sars-CoV-2.
Purification Method
Affinity purification
Gene ID
10410
RRID
AB_2861681
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using [KO Validated] IFITM3 Rabbit pAb (CAB13070) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and IFITM3 knockout (KO) HeLa cells, using [KO Validated] IFITM3 Rabbit pAb (CAB13070) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of C6 cells using [KO Validated] IFITM3 Rabbit pAb (CAB13070) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using [KO Validated] IFITM3 Rabbit pAb (CAB13070) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
