The IFN-beta Polyclonal Antibody (CAB23651) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables cytokine activity and type I interferon receptor binding activity. Involved in B cell proliferation; cellular response to virus; and type I interferon signaling pathway. Acts upstream of or within several processes, including cellular response to organic cyclic compound; defense response to other organism; and negative regulation of osteoclast differentiation. Located in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1). RRID Gene ID 15977 Swiss Prot Synonym Ifb; IFNB; If1da1; IFN-beta
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
IFN-beta Polyclonal Antibody
SKU:
CAB23651
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISAIF-P
Recommended Dilution:
WB
1:1000 - 1:5000
IF
/
ICC
1:50 - 1:200
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Ifb, IFNB, If1da1, IFN-beta
Positive Sample:
Recombinant Mouse IFN-beta Protein
Cellular Localization:
-
Calculated MW:
22kDa
Observed MW:
63kDa (C-hFc&His taged Active Recombinant Mouse IFN-beta Protein)，24kDa/26kDa(Endogenous)
Enables cytokine activity and type I interferon receptor binding activity. Involved in B cell proliferation; cellular response to virus; and type I interferon signaling pathway. Acts upstream of or within several processes, including cellular response to organic cyclic compound; defense response to other organism; and negative regulation of osteoclast differentiation. Located in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1). RRID Gene ID 15977 Swiss Prot Synonym Ifb; IFNB; If1da1; IFN-beta
Purification Method:
Affinity purification
Gene ID:
15977
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of Recombinant Mouse IFN-beta Protein (RP01076) using IFN-beta Rabbit pAb (CAB23651) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 10ng/5ng/1ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunofluorescence analysis of HepG2 cells using IFN-beta Rabbit pAb (CAB23651) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
