The IFNGR1 Monoclonal Antibody (CAB11653) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, exhibits high specificity and sensitivity for human samples, making it an excellent choice for Western blot applications.IFNGR1, also known as interferon gamma receptor 1, plays a crucial role in the activation of the immune system in response to pathogens. By binding to IFNGR1, researchers can accurately detect and analyze this important protein in various cell types, offering valuable insights into immune function and potential therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IFNGR1 Monoclonal Antibody
SKU:
CAB11653
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC50421
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD119, IFNGR, IMD27A, IMD27B, R1
Positive Sample:
293T, BxPC-3, Mouse stomach, Mouse thymus, Rat lung
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
54kDa
Observed MW:
45-90kDa
This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
Purification Method
Affinity purification
Gene ID
3459
RRID
AB_2861620
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using [KO Validated] IFNGR1 Rabbit mAb (CAB11653) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Western blot analysis of lysates from wild type(WT) and IFNGR1 knockout (KO) 293T cells, using [KO Validated] IFNGR1 Rabbit mAb (CAB11653) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Enhanced Kit (AbGn00021). Exposure time: 180s.
