The IGF1 Polyclonal Antibody (CAB24744) is a high-quality antibody developed for reliable detection and analysis of target proteins. IGF-1 (Insulin-like Growth Factor 1) is a secreted protein structurally and functionally similar to insulin, processed from a precursor and bound by specific receptors to mediate cell growth and development. Defects in this gene cause IGF-1 deficiency, a condition associated with growth retardation and developmental abnormalities.
This antibody is validated for use in WB applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
IGF1 Polyclonal Antibody
SKU:
CAB24744
Size:
100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WB
Recommended Dilution:
WB
,1:500 - 1:1000
Synonyms:
IGF, MGF, IGFI, IGF-I, IGF1
Positive Sample:
Mouse liver, Rat liver
Cellular Localization:
Secreted.
Calculated MW:
22kDa
Observed MW:
20kDa
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
Purification Method
Affinity purification
Gene ID
3479
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IGF1 Rabbit pAb (CAB24744) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
