The IGF2BP1/IMP1 Polyclonal Antibody (CAB22246) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IGF2BP1/IMP1 Polyclonal Antibody
SKU:
CAB22246
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IMP1, ZBP1, CRDBP, IMP-1, CRD-BP, VICKZ1, P1
Positive Sample:
HepG2, 293F, NIH/3T3, Mouse brain, Rat kidney
Cellular Localization:
Crd-Mediated Mrna Stability Complex, Cytoplasm, Cytoplasmic Stress Granule, Cytosol, Dendritic Spine, Growth Cone, Nucleoplasm, Nucleus, P-Body, Perinuclear Region Of Cytoplasm.
Calculated MW:
63kDa
Observed MW:
64kDa
This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
10642
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and knockdown (KD) HepG2 cells, using [KO Validated] IGF2BP1/IMP1 Rabbit pAb (CAB22246) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from 293F cells, using [KO Validated] IGF2BP1/IMP1 Rabbit pAb (CAB22246) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
