The IGF2BP3 Antibody (CAB4444) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in Western blotting applications. It binds specifically to IGF2BP3, allowing for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in molecular biology and cancer research.IGF2BP3, also known as insulin-like growth factor 2 mRNA-binding protein 3, plays a critical role in post-transcriptional regulation of gene expression and has been implicated in the progression of various cancers.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
IGF2BP3 Antibody
SKU:
CAB4444
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF/ICC
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KOC, CT98, IMP3, KOC1, IMP-3, VICKZ3, IGF2BP3
Positive Sample:
Hep G2, A549, 293T, HeLa
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
64kDa
Observed MW:
70kDa
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.
Purification Method
Affinity purification
Gene ID
10643
RRID
AB_2765679
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using IGF2BP3 Rabbit pAb (CAB4444) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunohistochemistry analysis of paraffin-embedded Human esophagus using IGF2BP3 Rabbit pAb (CAB4444) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using IGF2BP3 Rabbit pAb (CAB4444) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of U2OS cells using IGF2BP3 Rabbit pAb (CAB4444) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
