The Cation-independent M6PR (IGF2R) Antibody (CAB13022) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By targeting the IGF2R protein, this antibody enables researchers to detect and analyze IGF2R levels in various cell types, providing valuable insights into the role of IGF2R in cellular processes related to growth and development.IGF2R is a key receptor involved in the regulation of insulin-like growth factors, playing a crucial role in growth inhibition and tumor suppression. Studies have shown that dysregulation of IGF2R expression is associated with various diseases, including cancer and developmental abnormalities.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cation-independent M6PR (IGF2R) Antibody
SKU:
CAB13022
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Lysosome Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
274kDa
Observed MW:
274kDa
This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).
Purification Method
Affinity purification
Gene ID
3482
RRID
AB_2759869
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HepG2 cells, using Cation-independent M6PR (IGF2R) Rabbit pAb (CAB13022) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of C6 cells using Cation-independent M6PR (IGF2R) Rabbit pAb (CAB13022) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Cation-independent M6PR (IGF2R) Rabbit pAb (CAB13022) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
