Description
Anti-IL-10RA Antibody (CAB1830)
The IL-10RA Polyclonal Antibody (CAB1830) is specially designed for research involving IL-10RA, a critical receptor involved in the regulation of the immune system. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blotting.IL-10RA, also known as interleukin-10 receptor alpha, plays a crucial role in mediating the anti-inflammatory effects of interleukin-10 (IL-10) in the immune system. By binding to IL-10, IL-10RA helps regulate immune responses, promoting immune tolerance and reducing inflammation.
This makes IL-10RA a key target for research in immunology, inflammatory diseases, and autoimmune disorders.By using the IL-10RA Polyclonal Antibody (CAB1830), researchers can effectively study the expression and function of IL-10RA in different cell types and tissues. Understanding the role of IL-10RA in immune regulation can provide valuable insights for developing novel therapeutic approaches for immune-mediated diseases.
| Antibody Name: | IL10RA Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB1830 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human IL10RA (NP_001549.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, Raji, RAW264.7, Mouse thymus, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human IL10RA (NP_001549.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HGTE LPSP PSVW FEAE FFHH ILHW TPIP NQSE STCY EVAL LRYG IESW NSIS NCSQ TLSY DLTA VTLD LYHS NGYR ARVR AVDG SRHS NWTV TNTR FSVD EVTL TVGS VNLE IHNG FILG KIQL PRPK MAPA NDTY ESIF SHFR EYEI AIRK VPGN FTFT HKKV KHEN FSLL TSGE VGEF CVQV KPSV ASRS NKGM WSKE ECIS LTRQ Y |
| Gene ID: | 3587 |
| Uniprot: | Q13651 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 63kDa |
| Observed MW: | 90-110KDa |
| Synonyms: | IL10RA, CD210, CD210a, CDW210A, HIL-10R, IL-10R1, IL10R |
| Background: | The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. |
| UniProt Protein Function: | IL10RA: a receptor for interleukin 10 structurally related to interferon receptors. Mediates the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, cytokine Chromosomal Location of Human Ortholog: 11q23 Cellular Component: integral to membrane; plasma membrane Molecular Function:interleukin-10 binding; interleukin-10 receptor activity; protein binding; receptor activity; signal transducer activity Biological Process: cytokine and chemokine mediated signaling pathway; response to lipopolysaccharide Disease: Inflammatory Bowel Disease 28, Autosomal Recessive |
| NCBI Summary: | The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009] |
| UniProt Code: | Q13651 |
| NCBI GenInfo Identifier: | 322510034 |
| NCBI Gene ID: | 3587 |
| NCBI Accession: | Q13651.2 |
| UniProt Secondary Accession: | Q13651,A8K6I0, B0YJ27, |
| UniProt Related Accession: | Q13651 |
| Molecular Weight: | |
| NCBI Full Name: | Interleukin-10 receptor subunit alpha |
| NCBI Synonym Full Names: | interleukin 10 receptor subunit alpha |
| NCBI Official Symbol: | IL10RAÂ Â |
| NCBI Official Synonym Symbols: | CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1Â Â |
| NCBI Protein Information: | interleukin-10 receptor subunit alpha |
| UniProt Protein Name: | Interleukin-10 receptor subunit alpha |
| UniProt Synonym Protein Names: | CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; CD_antigen: CD210 |
| Protein Family: | Interleukin-10 receptor |
| UniProt Gene Name: | IL10RAÂ Â |
| UniProt Entry Name: | I10R1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IL10RA antibody (CAB1830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (CAB1830) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using IL10RA Antibody (CAB1830) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using IL10RA Antibody (CAB1830) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using IL10RA Rabbit pAb (CAB1830) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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