The IL12B Polyclonal Antibody (CAB23054) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it suitable for Western blot applications. By targeting the IL12B protein, this antibody enables researchers to detect and analyze IL-12 beta subunit expression in different cell types, making it ideal for studies in immunology and cancer research.IL-12 is a key player in the immune system, promoting the development of T helper 1 cells and enhancing the cytotoxicity of natural killer cells and cytotoxic T cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IL12B Polyclonal Antibody
SKU:
CAB23054
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p40, Il-12b, Il12p40, Il-12p40, IL12B
Positive Sample:
HeLa, U-87 MG, 293F
Calculated MW:
38kDa
Observed MW:
38kDa
This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23.
Purification Method
Affinity purification
Gene ID
16160
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using IL12B Rabbit pAb (CAB23054) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
