The IL-1RA Rabbit Monoclonal Antibody is a highly specific and sensitive tool for researchers studying interleukin-1 receptor antagonist (IL-1RA) in various biological samples. This antibody, raised in rabbits, is validated for use in applications such as Western blotting, immunofluorescence, and immunohistochemistry.IL-1RA is a natural antagonist of the pro-inflammatory cytokine interleukin-1 (IL-1), and plays a crucial role in regulating inflammatory responses and autoimmune disorders. By targeting IL-1RA with this monoclonal antibody, researchers can effectively study the role of IL-1RA in inflammation, immunology, and disease pathology.
With its high specificity and sensitivity, the IL-1RA Rabbit Monoclonal Antibody is an invaluable tool for scientists conducting research in areas such as immunology, inflammation, and autoimmune diseases. Its ability to detect and quantify IL-1RA protein levels in various sample types makes it an essential component in studies aimed at understanding the mechanisms of immune regulation and developing novel therapeutic strategies.
Product Name:
IL1RA Rabbit Monoclonal Antibody
SKU:
CAB11103
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-177 of human IL1RA (NP_776214.1)
Sequence:
ETRL QLEA VNIT DLSE NRKQ DKRF AFIR SDSG PTTS FESA ACPG WFLC TAME ADQP VSLT NMPD EGVM VTKF YFQE DE
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:
DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Positive Sample:
TE-1,A-431,NIH/3T3,SW480,Mouse lung,Mouse liver,Rat large intestine,Rat lung
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Secreted.
Calculated MW:
20kDa
Observed MW:
22kDa
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
3557
Clone Number:
ARC0524
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using IL1RA Rabbit mAb (CAB11103) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
