Description
IL4 Rabbit Polyclonal Antibody (CAB14660)
The Anti-IL4 Antibody (CAB14660) offered by Assay Genie is designed for research involving the interleukin-4 (IL-4) protein, a key player in immune regulation and inflammation. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it suitable for various applications such as Western blotting.IL-4 is a cytokine that plays a crucial role in the immune response, promoting differentiation of T cells and B cells, as well as regulating the activation of macrophages. Dysregulation of IL-4 has been implicated in a variety of inflammatory conditions and autoimmune disorders, making it a valuable target for research in immunology and disease.
With the Anti-IL4 Antibody, researchers can detect and analyze IL-4 expression in different cell types, aiding in the study of immune responses and the development of targeted therapies. This antibody is an essential tool for advancing our understanding of IL-4 function and its potential role in diseases such as allergies, asthma, and autoimmune conditions.
| Product Name: | IL4 Rabbit Polyclonal Antibody |
| SKU: | CAB14660 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL4 (NP_000580.1). |
| Sequence: | MGLT SQLL PPLF FLLA CAGN FVHG HKCD ITLQ EIIK TLNS LTEQ KTLC TELT VTDI FAAS KNTT EKET FCRA ATVL RQFY SHHE KDTR CLGA TAQQ FHRH |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | BSF1; IL-4; BCGF1; BSF-1; BCGF-1; IL4 |
| Positive Sample: | Mouse eye |
| Conjugate: | Unconjugated |
| Cellular Localization: | Secreted. |
| Calculated MW: | 17kDa |
| Observed MW: | 17kDa |
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
| Purification Method: | Affinity purification |
| Gene ID: | 3565 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from Mouse eye, using IL4 Rabbit pAb (CAB14660) at 1:700 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. |
