The Integrin beta 3 (ITGB3/CD61) Antibody (CAB2542) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the integrin beta-3 protein, this antibody enables detection and analysis in a variety of cell types, making it well-suited for investigations in cell biology, developmental biology, and cancer research.Integrin beta-3 plays a critical role in mediating cell adhesion to the extracellular matrix, as well as facilitating cell migration and invasion in various physiological and pathological processes.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Integrin beta 3 (ITGB3/CD61) Antibody
SKU:
CAB2542
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Junction, Cell Membrane, Cell Projection, Single-Pass Type I Membrane Protein, Focal Adhesion, Lamellipodium Membrane.
Calculated MW:
87kDa
Observed MW:
100kDa
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Purification Method
Affinity purification
Gene ID
3690
RRID
AB_2764431
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Integrin beta 3 (ITGB3/CD61) Rabbit pAb (CAB2542) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from HUVEC cells, using Integrin beta 3 (ITGB3/CD61) Rabbit pAb (CAB2542) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Mouse bone marrow using Integrin beta 3 (ITGB3/CD61) Rabbit pAb (CAB2542) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
