The IP3R Monoclonal Antibody (CAB4436) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using rabbit monoclonal technology, is highly specific and sensitive, making it ideal for use in various applications such as Western blot, immunofluorescence, and immunohistochemistry.The IP3R protein, also known as inositol trisphosphate receptor, is a critical component in the regulation of intracellular calcium levels, impacting cell signaling, apoptosis, and cell proliferation. Dysregulation of IP3R has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
IP3R Monoclonal Antibody
SKU:
CAB4436
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC1007
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene.
Purification Method
Affinity purification
Gene ID
3708
RRID
AB_2863274
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat brain using IP3R Rabbit mAb (CAB4436) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Western blot analysis of lysates from Mouse brain using IP3R Rabbit mAb (CAB4436) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using IP3R Rabbit mAb (CAB4436) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded rat brain using IP3R Rabbit mAb (CAB4436) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
