The IRF2 Monoclonal Antibody (CAB4843) is a high-quality antibody developed for reliable detection and analysis of target proteins. IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
IRF2 Monoclonal Antibody
SKU:
CAB4843
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC0265
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IRF-2, IRF2
Positive Sample:
HeLa, Ramos, A20
Cellular Localization:
Nucleus.
Calculated MW:
39kDa
Observed MW:
50kDa
IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4.
Purification Method
Affinity purification
Gene ID
3660
RRID
AB_2863358
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IRF2 Rabbit mAb (CAB4843) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg IRF2 antibody (CAB4843). Western blot was performed from the immunoprecipitate using IRF2 (CAB4843) at a dilution of 1:500.
