The Involucrin Monoclonal Antibody (CAB13311) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is ideal for use in immunohistochemistry and immunofluorescence applications, allowing for the visualization and analysis of involucrin expression in various tissue samples.Involucrin is a key protein involved in the terminal differentiation of keratinocytes, forming cross-linked structures that contribute to the structural integrity of the skin barrier. Its expression is essential for the formation of the cornified envelope, a protective layer that prevents water loss and protects against external insults.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Involucrin Monoclonal Antibody
SKU:
CAB13311
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1802
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
Purification Method
Affinity purification
Gene ID
3713
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using Involucrin Rabbit mAb (CAB13311) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Involucrin Rabbit mAb (CAB13311) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
