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Anti-JAK3 Antibody (CAB0748)

SKU:
CAB0748
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 1 Anti-JAK3 Antibody CAB0748
  • Immunology Antibodies 1 Anti-JAK3 Antibody CAB0748
  • Immunology Antibodies 1 Anti-JAK3 Antibody CAB0748
$167 - $503
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Description

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Antibody Name:Anti-JAK3 Antibody
Antibody SKU:CAB0748
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 750-850 of human JAK3 (NP_000206.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 750-850 of human JAK3 (NP_000206.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TELA LLIQ QCMA YEPV QRPS FRAV IRDL NSLI SSDY ELLS DPTP GALA PRDG LWNG AQLY ACQD PTIF EERH LKYI SQLG KGNF GSVE LCRY DPLG DNTG A
Gene ID:3718
Uniprot:P52333
Cellular Location:Cytoplasm, Endomembrane system, Peripheral membrane protein
Calculated MW:68kDa/121kDa/125kDa
Observed MW:115KDa
Synonyms:JAK3, JAK-3, JAK3_HUMAN, JAKL, L-JAK, LJAK
Background:The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).
UniProt Protein Function:JAK3: a non-receptor tyrosine-kinase of the Jak family expressed predominantly in immune cells. Mediates signal transduction via the common gamma-chain of cytokine receptors, including IL-2, -4, -7, -9, and -15. Interacts with members of the STAT (signal transduction and activators of transcription) family. Interacts with STAM2 and SHB. Contains two protein kinase domains. Mutations that abrogate JAK3 function cause an autosomal SCID (severe combined immunodeficiency disease). Inhibitor: R017s for organ transplants. Three differentially spliced isoforms have been described.
UniProt Protein Details:

Protein type:EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; JakA family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: cytoskeleton; cytosol; endomembrane system; extrinsic to internal side of plasma membrane

Molecular Function:ATP binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein phosphatase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding

Biological Process: adaptive immune response; B cell differentiation; cell migration; elevation of cytosolic calcium ion concentration; enzyme linked receptor protein signaling pathway; innate immune response; MAPKKK cascade; negative regulation of dendritic cell cytokine production; negative regulation of FasL biosynthetic process; negative regulation of interleukin-10 production; negative regulation of interleukin-12 production; negative regulation of T cell activation; negative regulation of T-helper 1 cell differentiation; peptidyl-tyrosine phosphorylation; positive regulation of activated T cell proliferation; positive regulation of calcium ion transport; positive regulation of GTPase activity; positive regulation of immune response; positive regulation of T cell proliferation; protein amino acid phosphorylation; STAT protein nuclear translocation; T cell homeostasis; tyrosine phosphorylation of STAT protein; tyrosine phosphorylation of Stat5 protein

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-negative

NCBI Summary:The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]
UniProt Code:P52333
NCBI GenInfo Identifier:50403745
NCBI Gene ID:3718
NCBI Accession:P52333.2
UniProt Secondary Accession:P52333,Q13259, Q13260, Q13611, Q8N1E8, Q99699, Q9Y6S2
UniProt Related Accession:P52333
Molecular Weight:
NCBI Full Name:Tyrosine-protein kinase JAK3
NCBI Synonym Full Names:Janus kinase 3
NCBI Official Symbol:JAK3  
NCBI Official Synonym Symbols:JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN  
NCBI Protein Information:tyrosine-protein kinase JAK3
UniProt Protein Name:Tyrosine-protein kinase JAK3
UniProt Synonym Protein Names:Janus kinase 3; JAK-3; Leukocyte janus kinase; L-JAK
Protein Family:Tyrosine-protein kinase
UniProt Gene Name:JAK3  
UniProt Entry Name:JAK3_HUMAN
Anti-JAK3 Antibody (CAB0748)Western blot analysis of extracts of Jurkat cells, using JAK3 antibody (CAB0748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-JAK3 Antibody (CAB0748)Immunofluorescence analysis of Raw264.7 cells using JAK3 Polyclonal Antibody (CAB0748) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-JAK3 Antibody (CAB0748)Immunofluorescence analysis of THP-1 cells using JAK3 Polyclonal Antibody (CAB0748) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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