The c-Jun Antibody (CAB0246) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in various applications, including Western blot and immunohistochemistry. By binding specifically to the JUN protein, this antibody allows for the detection and analysis of JUN expression in a wide range of cell types.JUN is a key player in gene regulation, controlling the expression of genes involved in processes such as cell growth, survival, and response to stress.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
c-Jun Antibody
SKU:
CAB0246
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AP1, p39, AP-1, cJUN, c-Jun
Positive Sample:
HeLa, HT-1080, NIH/3T3
Cellular Localization:
Nucleus.
Calculated MW:
36kDa
Observed MW:
46kDa/42kDa
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Purification Method
Affinity purification
Gene ID
3725
RRID
AB_2757059
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using c-Jun Rabbit pAb (CAB0246) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of NIH/3T3 cells using c-Jun Rabbit pAb (CAB0246) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
