The JunD Monoclonal Antibody (CAB5433) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216).
This antibody is validated for use in WB, ChIP, ELISA, CUT&Tag applications and has demonstrated reactivity against Human samples.
Product Name:
JunD Monoclonal Antibody
SKU:
CAB5433
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC1408
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBChIPELISACUT&Tag
Recommended Dilution:
WB
1:1000 - 1:6000
ChIP
5μg antibody for 10μg-15μg of Chromatin
CUT&Tag
10⁵ cells /1 μg
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AP-1, JunD
Positive Sample:
HeLa, HT-1080
Cellular Localization:
Nucleus.
Calculated MW:
35 kDa
Observed MW:
38 kDa/42 kDa
The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216).
Purification Method
Affinity purification
Gene ID
3727
RRID
AB_2863501
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using JunD Rabbit mAb (CAB5433) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using JunD antibody (CAB5433) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
