K6-linkage Specific Polyubiquitin Antibody (CAB18106)
The K6-linkage Specific Polyubiquitin Antibody (CAB18106) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for K6-linked polyubiquitin chains in human samples, making it an ideal choice for Western blot applications.K6 linkage-specific polyubiquitin chains play a critical role in protein degradation, signal transduction, and DNA repair processes. By targeting these specific chains, researchers can gain valuable insights into the mechanisms underlying these cellular processes.
This antibody is validated for use in WB, ELISA, DB applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
K6-linkage Specific Polyubiquitin Antibody
SKU:
CAB18106
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISADB
Recommended Dilution:
WB
1:500 - 1:2000
DB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
RAW264.7, C6
Polyubiquitin chains regulate diverse cellular processes through the ability of ubiquitin to form chains of eight different linkage types. Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair. it has been reported that modification at Lys-6 inhibits ubiquitin-dependent protein degradation, a failure of the ubiquitin-proteasome system could play a role in initiating the formation of degradation-resistant PHF tangles.
Purification Method
Affinity purification
RRID
AB_2861898
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using K6-linkage Specific Polyubiquitin Rabbit pAb (CAB18106) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Dot-blot analysis of all sorts of peptides using K6-linkage Specific Polyubiquitin antibody (CAB18106) at 1:1000 dilution.
