The KD validated PROM1 polyclonal antibody is designed for research involving the PROM1 protein, also known as CD133, a cell surface marker commonly found on stem cells, cancer cells, and endothelial cells. This antibody, raised in rabbits, is highly specific and sensitive to PROM1, making it ideal for applications such as immunohistochemistry and flow cytometry.PROM1 plays a crucial role in stem cell biology, tumor initiation, and cancer progression.
Its expression has been linked to cancer stem cells and resistance to therapy, making it a valuable target for cancer research and personalized medicine approaches. The KD validated PROM1 polyclonal antibody enables accurate detection and analysis of PROM1 expression in various cell types, providing valuable insights into its function and potential as a therapeutic target.
Product Name:
[KD Validated] PROM1 Polyclonal Antibody
SKU:
CAB21701
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human PROM1 (NP_006008.1).
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8842
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29 cells, using [KD Validated] PROM1 Rabbit pAb (CAB21701) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
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