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[KD Validated] PROM1 Polyclonal Antibody (CAB21701)

SKU:
CAB21701
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IHC
IP
IF
€139 - €529
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KD Validated] PROM1 Polyclonal Antibody
SKU:CAB21701
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human PROM1 (NP_006008.1).
Sequence:ANHQ VRTR IKRS RKLA DSNF KDLR TLLN ETPE QIKY ILAQ YNTT KDKA FTDL NSIN SVLG GGIL DRLR PNII PVLD EIKS MATA IKET KEAL ENMN STLK SLHQ QSTQ LSSS LTSV KTSL RSSL NDPL CLVH PSSE TCNS IRLS LSQL NSNP ELRQ LPPV DAEL DNVN NVLR TDLD GLVQ QGYQ SLND IPDR VQRQ TTTV VAGI KRVL NSIG SDID NVTQ RLPI QDIL SAFS VYVN NTES YIHR NLPT LEEY DSY
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsIF/ICC,1:50 - 1:200
Synonyms:RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; [KD Validated] PROM1
Positive Sample:HT-29,HCT116
Conjugate:Unconjugated
Cellular Localization:Apical cell membrane, Cell projection, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Multi-pass membrane protein, cilium, microvillus membrane, photoreceptor outer segment.
Calculated MW:97kDa
Observed MW:120kDa

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:8842
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HT-29 cells, using [KD Validated] PROM1 Rabbit pAb (CAB21701) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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