The KDM4B/JMJD2B Antibody (CAB5824) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications, enabling accurate detection and analysis of KDM4B protein levels in various cell types.KDM4B, also known as lysine-specific demethylase 4B, plays a crucial role in chromatin remodeling and transcriptional regulation, making it a key player in cellular processes such as DNA replication and cell cycle progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
KDM4B/JMJD2B Antibody
SKU:
CAB5824
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MRD65, JMJD2B, TDRD14B, KDM4B/JMJD2B
Positive Sample:
Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
122kDa
Observed MW:
150kDa
Enables histone H3-methyl-lysine-36 demethylase activity and histone H3-methyl-lysine-9 demethylase activity. Involved in histone H3-K36 demethylation and histone H3-K9 demethylation. Located in cytosol and nucleoplasm. Implicated in autosomal dominant non-syndromic intellectual disability; breast cancer; colorectal cancer; malignant peripheral nerve sheath tumor; and stomach cancer. Biomarker of several diseases, including alopecia areata; lung cancer; medulloblastoma; prostate cancer; and stomach cancer.
Purification Method
Affinity purification
Gene ID
23030
RRID
AB_2766576
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain, using (CAB5824) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
