The KDM4B/JMJD2B Monoclonal Antibody (CAB6670) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables histone H3-methyl-lysine-36 demethylase activity and histone H3-methyl-lysine-9 demethylase activity. Involved in histone H3-K36 demethylation and histone H3-K9 demethylation. Located in cytosol and nucleoplasm. Implicated in autosomal dominant non-syndromic intellectual disability; breast cancer; colorectal cancer; malignant peripheral nerve sheath tumor; and stomach cancer. Biomarker of several diseases, including alopecia areata; lung cancer; medulloblastoma; prostate cancer; and stomach cancer.
This antibody is validated for use in WB, IF/ICC, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KDM4B/JMJD2B Monoclonal Antibody
SKU:
CAB6670
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1416
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPChIPELISA
Recommended Dilution:
WB
1:3000 - 1:20000
IF/ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 10μg-15μg of Chromatin
Synonyms:
MRD65, JMJD2B, TDRD14B, KDM4B/JMJD2B
Positive Sample:
T-47D, HCT 116
Cellular Localization:
Nucleus.
Calculated MW:
122 kDa
Observed MW:
150 kDa
Enables histone H3-methyl-lysine-36 demethylase activity and histone H3-methyl-lysine-9 demethylase activity. Involved in histone H3-K36 demethylation and histone H3-K9 demethylation. Located in cytosol and nucleoplasm. Implicated in autosomal dominant non-syndromic intellectual disability; breast cancer; colorectal cancer; malignant peripheral nerve sheath tumor; and stomach cancer. Biomarker of several diseases, including alopecia areata; lung cancer; medulloblastoma; prostate cancer; and stomach cancer.
Purification Method
Affinity purification
Gene ID
23030
RRID
AB_2863534
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from T-47D cells using KDM4B/JMJD2B Rabbit mAb (CAB6670) at 1:7000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5 s.
Immunofluorescence analysis of C6 cells using KDM4B/JMJD2B Rabbit mAb (CAB6670) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of HCT 116 cells using 3 μg KDM4B/JMJD2B Rabbit mAb (CAB6670). Western blot was performed from the immunoprecipitate using KDM4B/JMJD2B Rabbit mAb (CAB6670) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of 5637 cells, using KDM4B/JMJD2B Rabbit mAb (CAB6670) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
