The KLF1 Antibody (CAB10581) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.KLF1 is known for its involvement in the development and differentiation of red blood cells, making it a key player in hemoglobin synthesis and erythrocyte maturation. By targeting the KLF1 protein, researchers can detect and analyze its expression in various cell types, providing valuable insights into its function in normal and diseased states.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
KLF1 Antibody
SKU:
CAB10581
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ALGP FPDT QDDF LKWW RSE
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EKLF, EKLF/KLF1, KLF1
Positive Sample:
Rat plasma
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
38kDa
This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype.
Purification Method
Affinity purification
Gene ID
10661
RRID
AB_2758122
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat plasma, using KLF1 Rabbit pAb (CAB10581) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
