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KLHL20 Antibody (PACO59005)

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SKU:
PACO59005
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IHC
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • KLHL20 Antibody PACO59005
  • KLHL20 Antibody PACO59005
  • KLHL20 Antibody PACO59005
€339

Description

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KLHL20 Antibody (PACO59005)

The KLHL20 Polyclonal Antibody (PACO59005) is a vital tool for researchers studying the KLHL20 protein, known for its involvement in cellular processes such as protein degradation and cell migration. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to KLHL20, it enables precise detection and analysis in a variety of cell types, making it an excellent choice for studies in cell biology and cancer research.KLHL20 has been identified as a potential regulator of cancer progression and metastasis, making it a promising target for therapeutic interventions in cancer treatment.

Its role in influencing key cellular functions highlights the importance of further research into its mechanisms and potential implications for cancer therapies. Utilizing the KLHL20 Polyclonal Antibody can provide valuable insights into the function and behavior of KLHL20, ultimately contributing to the development of novel strategies for combating cancer.

Antibody Name:KLHL20 Antibody (PACO59005)
Antibody SKU:PACO59005
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, IHC, IF
Recommended Dilutions:ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:Human
Immunogen:Recombinant Human Kelch-like protein 20 protein (1-200AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageIHC image of PACO59005 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product ImageImmunofluorescence staining of Hela cells with PACO59005 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product ImageIHC image of PACO59005 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination. Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11. In case of tumor, the BCR(KLHL20) E3 ubiquitin ligase complex is involved in tumor hypoxia: following hypoxia, the BCR(KLHL20)complex mediates ubiquitination and degradation of PML, potentiating HIF-1 signaling and cancer progression.
Synonyms:Kelch-like protein 20 (Kelch-like ECT2-interacting protein) (Kelch-like protein X), KLHL20, KLEIP KLHLX
UniProt Protein Function:Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis (PubMed:20389280). The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination (PubMed:24768539). Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking (PubMed:24768539). Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (PubMed:21670212). In case of tumor, the BCR(KLHL20) E3 ubiquitin ligase complex is involved in tumor hypoxia: following hypoxia, the BCR(KLHL20)complex mediates ubiquitination and degradation of PML, potentiating HIF-1 signaling and cancer progression (PubMed:21840486).
UniProt Protein Details:
NCBI Summary:The protein encoded by this gene is a member of the kelch family of proteins, which is characterized by a 44-56 amino acid repeat motif. The kelch motif appears in many different polypeptide contexts and contains multiple potential protein-protein contact sites. Members of this family are present both throughout the cell and extracellularly, with diverse activities. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y2M5
NCBI GenInfo Identifier:40807500
NCBI Gene ID:27252
NCBI Accession:NP_055273.2
UniProt Secondary Accession:Q9Y2M5,Q5TZF2, Q5ZF45, Q9H457, B3KMA0, B4DUR0
UniProt Related Accession:Q9Y2M5
Molecular Weight:46,490 Da
NCBI Full Name:kelch-like protein 20
NCBI Synonym Full Names:kelch like family member 20
NCBI Official Symbol:KLHL20  
NCBI Official Synonym Symbols:KHLHX; KLEIP; KLHLX  
NCBI Protein Information:kelch-like protein 20
UniProt Protein Name:Kelch-like protein 20
UniProt Synonym Protein Names:Kelch-like ECT2-interacting protein; Kelch-like protein X
Protein Family:Kelch-like protein
UniProt Gene Name:KLHL20  
UniProt Entry Name:
Antibodies
KLHL20 Antibody (PACO10134)
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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