The KLRC3 Polyclonal Antibody (PACO59948) is a vital tool for research involving KLRC3, a cell surface molecule with important implications in immune regulation. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding specifically to the KLRC3 protein, this antibody allows for the detection and analysis of KLRC3 in various cell types, making it an ideal choice for studies in immunology and cancer research.KLRC3, also known as natural killer cell lectin-like receptor subfamily C member 3, plays a crucial role in the immune response by modulating the function of natural killer cells.
Its involvement in immune regulation makes it a key target for research into diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. Understanding the function of KLRC3 is essential for the development of therapies that aim to harness or modulate the immune system in these disease contexts.In summary, the KLRC3 Polyclonal Antibody (PACO59948) is a valuable tool for researchers studying immune regulation, particularly in the context of cancer and inflammatory diseases. Its specificity, reliability, and validated performance in Western blot applications make it an indispensable asset in the field of immunology research.
IHC image of PACO59948 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO59948 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
Synonyms:
NKG2-E type II integral membrane protein (NK cell receptor E) (NKG2-E-activating NK receptor), KLRC3, NKG2E
UniProt Protein Function:
KLRC3: Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 12p13Cellular Component: integral to membraneMolecular Function: transmembrane receptor activity; carbohydrate bindingBiological Process: cellular defense response; signal transduction
UniProt Protein Details:
NCBI Summary:
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. KLRC3 is a member of the NKG2 group which are expressed primarily in natural killer (NK) cells and encodes a family of transmembrane proteins characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed on NK cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
