[KO Validated] Annexin A5/Annexin V Antibody (CAB1728)
The [KO Validated] Annexin A5/Annexin V Antibody (CAB1728) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been rigorously validated for use in Western blot applications.Annexin A5 is a calcium-dependent phospholipid-binding protein that plays a crucial role in membrane trafficking, cell signaling, and cell death pathways. It is implicated in various cellular processes, including clotting, inflammation, and cancer progression.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] Annexin A5/Annexin V Antibody
SKU:
CAB1728
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PP4, ANX5, ENX2, CPB-I, RPRGL3, HEL-S-7, VAC-alph, V
Positive Sample:
HepG2, HeLa, DU145, PC-3, Mouse lung
Cellular Localization:
Cytoplasm, Cytosol, External Side Of Plasma Membrane, Extracellular Exosome, Extracellular Region, Focal Adhesion.
Calculated MW:
36kDa
Observed MW:
35kDa
The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications.
Purification Method
Affinity purification
Gene ID
308
RRID
AB_2861732
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] Annexin A5/Annexin V Rabbit pAb (CAB1728) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of lysates from wild type (WT) and Annexin A5/Annexin V knockout (KO) 293T cells, using [KO Validated] Annexin A5/Annexin V Rabbit pAb (CAB1728) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
