The APEH Polyclonal Antibody (CAB19912) is a vital tool for researchers studying the APEH protein, an enzyme involved in various cellular processes, including protein degradation and regulation of gene expression. This rabbit-raised antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the APEH protein, this antibody enables accurate detection and analysis in a range of cell types, making it essential for research in biology and biochemistry.
APEH, also known as Acylamino-acid-releasing enzyme, plays a crucial role in maintaining cellular homeostasis by regulating protein turnover and metabolism. Its diverse functions make it a promising target for investigating diseases such as neurodegenerative disorders, cancer, and metabolic syndromes. Understanding the role of APEH in cellular physiology is essential for developing new therapeutic approaches that target its activity in disease contexts.
Product Name:
[KO Validated] APEH Rabbit Polyclonal Antibody
SKU:
CAB19912
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3).
This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
327
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit pAb (CAB19912) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
