The KO Validated Casp1 Polyclonal Antibody (CAB18646) is a key tool for researchers studying caspase-1 (Casp1) protein, a crucial component in the inflammatory response and immune system regulation. This antibody, produced in rabbits, has been extensively validated for its specificity and sensitivity in detecting Casp1 in human samples, making it a reliable choice for Western blot applications.Casp1 is a pivotal enzyme involved in initiating inflammatory responses by cleaving pro-inflammatory cytokines such as pro-IL-1β and pro-IL-18. Dysregulation of Casp1 activity has been implicated in various diseases, including autoinflammatory disorders, infectious diseases, and neurodegenerative conditions.
Therefore, precise detection and analysis of Casp1 expression are essential for understanding its role in disease pathology and developing targeted therapeutic strategies.By utilizing the KO Validated Casp1 Polyclonal Antibody, researchers can effectively investigate the expression levels and activity of Casp1 in different cell types and disease models. This antibody's specificity and reliability make it a valuable tool for advancing research in immunology, inflammation, and therapeutic development aimed at modulating Casp1 activity for therapeutic benefit.
Product Name:
[KO Validated] CASP1 Rabbit Polyclonal Antibody
SKU:
CAB18646
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 198-297 of human CASP1 (NP_150634.1).
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
834
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CASP1 Rabbit pAb (CAB18646) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
