The [KO Validated] DIAPH1 Antibody (CAB18081) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is validated for use in various applications, including Western blot, immunofluorescence, and immunohistochemistry. It specifically targets the DIAPH1 protein, allowing for precise detection and analysis in a variety of cell types.DIAPH1 plays a critical role in cell migration, cell polarity, and cytokinesis, making it an important protein in cellular processes such as tumor invasion and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] DIAPH1 Antibody
SKU:
CAB18081
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1729
RRID
AB_2861875
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and DIAPH1 knockdown (KD) 293T cells, using [KD Validated] DIAPH1 Rabbit pAb (CAB18081) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
