The Fatty Acid Synthase (FASN) Antibody (CAB0461) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot applications. It binds specifically to the Fatty Acid Synthase protein, allowing for accurate detection and analysis in various cell types.Fatty Acid Synthase is a key enzyme involved in the de novo synthesis of long-chain fatty acids, a process essential for cellular function and energy storage. Dysregulation of fatty acid metabolism has been implicated in various diseases, including cancer, obesity, and metabolic disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Fatty Acid Synthase (FASN) Antibody
SKU:
CAB0461
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FAS, OA-519, SDR27X1, Fatty Acid Synthase (FASN)
Positive Sample:
MCF7, A-549, HeLa, 293T, Mouse liver, Mouse thymus, Rat liver
Cellular Localization:
Cytoplasm, Melanosome.
Calculated MW:
273kDa
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Purification Method
Affinity purification
Gene ID
2194
RRID
AB_2757202
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Fatty Acid Synthase (FASN) Rabbit pAb (CAB0461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates, using Fatty Acid Synthase (FASN) Rabbit pAb (CAB0461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.5s.
Western blot analysis of lysates from MCF7 cells, using Fatty Acid Synthase (FASN) Rabbit pAb (CAB0461) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.5s.
Western blot analysis of various lysates, using Fatty Acid Synthase (FASN) Rabbit pAb (CAB0461) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.5s.
Western blot analysis of lysates from wild type (WT) and Fatty Acid Synthase (FASN) knockout (KO) Hela(KO) cells, using Fatty Acid Synthase (FASN) (CAB0461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.5s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Fatty Acid Synthase (FASN) Rabbit pAb (CAB0461) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
