The [KO Validated] IL18 Antibody (CAB1115) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2861508 Gene ID 3606 Swiss Prot Synonym IGIF; IL-18; IL-1g; IL1F4; IL18
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] IL18 Antibody
SKU:
CAB1115
Size:
100μL, 20μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IGIF, IL-18, IL-1g, IL1F4, IL18
Positive Sample:
Recombinant Human IL-18 Protein
Cellular Localization:
Secreted.
Calculated MW:
22kDa
Observed MW:
18kDa
The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2861508 Gene ID 3606 Swiss Prot Synonym IGIF; IL-18; IL-1g; IL1F4; IL18
Purification Method
Affinity purification
Gene ID
3606
RRID
AB_2861508
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of Recombinant Human IL-18 Protein (RP00006) using IL18 Rabbit pAb (CAB1115) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 10 ng/1 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg IL18 antibody (CAB1115). Western blot was performed from the immunoprecipitate using IL18 antibody (CAB1115) at a dilution of 1:1000.
