The [KO Validated] Lamin B1 Antibody (CAB1910) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. RRID AB_2862592 Gene ID 4001 Swiss Prot Synonym LMN; ADLD; LMN2; LMNB; MCPH26; Lamin B1
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] Lamin B1 Antibody
SKU:
CAB1910
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPChIPELISA
Recommended Dilution:
WB
1:500 - 1:5000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF
/
ICC
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. Ch
IP
5μg antibody for 10μg-15μg of Chromatin
Synonyms:
LMN, ADLD, LMN2, LMNB, MCPH26, Lamin B1
Positive Sample:
HeLa, MCF7, Jurkat, Jurkat treated with Etoposide, L929, L929 treated with staurosporine, Mouse brain, Rat testis
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. RRID AB_2862592 Gene ID 4001 Swiss Prot Synonym LMN; ADLD; LMN2; LMNB; MCPH26; Lamin B1
Purification Method:
Affinity purification
Gene ID:
4001
RRID:
AB_2862592
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Lamin B1 Rabbit pAb (CAB1910) at 1:1000 dilution. Jurkat cells were treated with Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using Lamin B1 Rabbit pAb (CAB1910) at 1:1000 dilution. L929 cells were treated with staurosporine(1 uM) for 3 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Lamin B1 Rabbit pAb (CAB1910) at dilution of 1:150 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Lamin B1 Rabbit pAb (CAB1910) at dilution of 1:150 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 Rabbit pAb (CAB1910) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using Lamin B1 Rabbit pAb (CAB1910) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Lamin B1 Rabbit pAb (CAB1910) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Lamin B1 antibody (CAB1910). Western blot was performed from the immunoprecipitate using Lamin B1 antibody (CAB1910) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Lamin B1 Rabbit pAb antibody (CAB1910) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
