The [KO Validated] NDUFS3 Antibody (CAB8013) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency. RRID AB_2863565 Gene ID 4722 Swiss Prot Synonym CI-30; MC1DN8; NDUFS3
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] NDUFS3 Antibody
SKU:
CAB8013
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CI-30, MC1DN8, NDUFS3
Positive Sample:
HepG2, Mouse kidney, Mouse heart, Mouse skeletal muscle, Rat kidney, Rat heart, Rat skeletal muscle, MCF7, Rat heart
Cellular Localization:
Mitochondrion Inner Membrane.
Calculated MW:
30kDa
Observed MW:
26kDa/30kDa
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency. RRID AB_2863565 Gene ID 4722 Swiss Prot Synonym CI-30; MC1DN8; NDUFS3
Purification Method:
Affinity purification
Gene ID:
4722
RRID:
AB_2863565
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NDUFS3 Rabbit pAb (CAB8013) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunoprecipitation analysis of 600 μg extracts of Mouse kidney using 3 μg NDUFS3 antibody (CAB8013). Western blot was performed from the immunoprecipitate using NDUFS3 antibody (CAB8013) at a dilution of 1:1000.
