The [KO Validated] p53 Monoclonal Antibody (CAB19585) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been rigorously tested for knockout validation, ensuring its specificity and accuracy in detecting p53 in knockout cell lines.With its high reactivity in human samples, the p53 antibody is an essential tool for Western blot applications, allowing for precise analysis of p53 expression levels in various experimental settings. Its ability to bind specifically to the p53 protein makes it a reliable option for researchers investigating the role of p53 in cancer development, progression, and treatment.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] p53 Monoclonal Antibody
SKU:
CAB19585
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC56085
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method
Affinity purification
Gene ID
7157
RRID
AB_2862683
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb (CAB19585) at 1:10000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KO Validated] p53 Rabbit mAb (CAB19585). Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb (CAB19585) at a dilution of 1:1000.
