The [KO Validated] SETD7 Antibody (CAB9985) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly specific and sensitive for detecting SETD7 in human samples, making it suitable for various applications such as Western blotting.SETD7, also known as Histone-lysine N-methyltransferase SETD7, is a key player in epigenetic regulation and has been implicated in various biological processes including cell proliferation, differentiation, and development.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] SETD7 Antibody
SKU:
CAB9985
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KMT7, SET7, SET9, SET7/9, D7
Positive Sample:
293T
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
41kDa
Observed MW:
48kDa
Enables histone-lysine N-methyltransferase activity and p53 binding activity. Involved in peptidyl-lysine dimethylation and peptidyl-lysine monomethylation. Acts upstream of or within cellular response to DNA damage stimulus and heterochromatin organization. Located in chromosome and nucleolus.
Purification Method
Affinity purification
Gene ID
80854
RRID
AB_2863782
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and SETD7 knockout (KO) 293T cells, using [KO Validated] SETD7 Rabbit pAb (CAB9985) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
